Information on the EQA test Interphase-FISH Tumour
Aim
The objective of the interlaboratory test is to evaluate the technical performance of the FISH technique, the microscopic evaluation, the reporting and the interpretation of the FISH results in order to simulate a real diagnostic setting.
Performance interval
Once per year.
Procedure
1-ml cryotubes with the cell suspension fixed in methanol-acetic acid is sent out together with clinical information of a fictitious patient and a diagnostic question. Under normal transport conditions by mail (shipping to destinations outside of Germany by courier service), aliquoted samples are stable for up to 5 days. The diagnostic question and therewith the FISH probes that have to be applied change. As soon as the application period opens the FISH probes necessary for the upcoming interlaboratory test are announced.
Method
The FISH test should be carried out according to the own laboratory SOP. A diagnostic report has to be provided which includes the result of the FISH analysis and the interpretation in relation to the diagnostic question.
Criteria
Correctness of the qualitative and quantitative result and of the overall findings.
Certificate
A certificate will be provided that states whether or not the criteria according to RiLiBÄK are fulfilled and whether or not the participation was successful (correctness of the qualitative FISH result). The certificate is valid for two years.
EQA test supervisor
Prof. Dr. med. Claudia Haferlach
MLL Münchner Leukämie Labor GmbH
Max-Lebsche-Platz 31
81377 München
MLL Münchner Leukämie Labor GmbH
Max-Lebsche-Platz 31
81377 München
Dipl.-Biol. Jinda Holzwarth
MLL Münchner Leukämie Labor GmbH
Max-Lebsche-Platz 31
81377 München
If you would like to contact the EQA test supervisor(s), please call or e-mail the Coordination Office QA of BVDH:
Phone: +49 2403 83 80 54
E-mail: brandt@bvdh.de
Status as of: April/2025